Assay Services – What’s new?

AMITA KAPOOR BECOMES HEAD OF ASSAY SERVICES

November 2019

Amita Kapoor succeeds Toni Ziegler as head of the WNPRC Assay Services Unit. Dr. Kapoor completed both her undergraduate degree (Biology and Psychology) and Ph.D. (Physiology) at the University of Toronto. She has been part of Assay Services since 2011, leading the transition to state-of-the-art LC/MS/MS methods. Amita’s research focuses on using non-invasive measures to assess the relationship of hormone exposure during pregnancy to behaviors during the postnatal period.

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TARGETED PANELS FOR SPECIFIC STEROID TYPES BY LC/MS/MS

Target panels include metabolic pathways for androgens, estrogens, progestins or glucocorticoids. A comprehensive panel is available measuring the following steroids: cortisol, testosterone, androstenedione, DHEA, progesterone, estradiol, and estrone.

*Steroid panels may be customized by request from the investigator but may require further R&D.

Extraction methods are available for the following media: serum, plasma, urine, saliva, feces, cell extractions, and hair.

VITAMIN D ANALYSIS FOR HUMAN AND NON-HUMAN PRIMATES BY LC/MS/MS

Assay Services now offers measurement for:

  • 25-hydroxy vitamin D2&3: A rapid method requiring only 50 µl of serum for human samples and for nonhuman primates. NIST (NIH Vit. D Metabolites Quality Assurance Program) parcipitation ensures our values match the national standard.
  • 1,25-dihydroxyvitamin D2&3: Validated against RIA for humans samples and shows increased sensitivity over other methods using derivitazation. Measurements have been performed for humans using 500 µl, and less for the common marmoset.
  • Vitamin D metabolite panel: Cholecalciferol (D3), 24,25 dihydroxyvitamin D3, 24,25 dihydroxyvitamin D2, 25 hydroxyvitamin D3, 25 hydroxyvitamin D2.