Virology Services

The WNPRC Virology Services Unit provides expert support to investigators performing virological research in nonhuman primates. Our services include highly sensitive molecular diagnostics, production and titration of virus stocks suitable for in-vitro and/or in-vivo studies, generation of custom virus mutants by reverse genetics, virus isolation and more. Our clients include investigators studying the nonhuman primate AIDS viruses simian immunodeficiency virus (SIV) and simian-human immunodeficiency virus (SHIV), influenza, Zika virus and dengue, among other viruses.

SERVICES SUPPORTED

• Detection of viral RNA or DNA by ultra-sensitive quantitative RT-PCR (RT-qPCR) or quantitative PCR (qPCR). Viral targets include
  • SIV/SHIV
  • Simultaneous quantification of wild-type SIV and nef deletion mutants
  • Cytomegalovirus (from rhesus and cynomolgus macaques)
  • Mpox clade IIb
  • Influenza viruses
  • Zika virus
  • Dengue virus
  • Yellow fever virus 17D
  • Simian arteriviruses
  • SARS-CoV-2
  • Simian parvoviruses
• Production of characterized virus stocks:
  • Viruses can be purchased from existing stocks on a per-vial basis. We can also produce large-scale stocks to order. Please contact Virology Services Unit Head Thomas Friedrich, Ph.D. or virologyservices@lists.wisc.edu for more information.
  • Download information about a Zika virus stock for Puerto Rican isolate PRVABC59 (pdf) used in Zika virus studies at WNPRC.
  • View sample information sheets on SIV stocks, influenza and Mpox clade IIb
• Production of custom SIV mutants
• Isolation of nonhuman primate viruses in tissue culture
• Assays to measure the latent reservoir of SIV:
  • Intact proviral DNA assay (IPDA)
    • view IPDA white paper
  • Quantitative viral outgrowth assay
    • view QVOA white paper.pdf
  • Quantification of cell-associated SIV RNA or DNA by RT-qPCR/qPCR

For more information, please contact Virology Services Unit Head Thomas Friedrich, Ph.D. or virologyservices@lists.wisc.edu

To request service, please complete the Virology Services Service Request Form.

PRICING INFORMATION

Virology Services pricing as of 5/1/24

Left: SCV2 Life Cycle Illustration by David S. Goodsell, RCSB Protein Data Bank; doi: 10.2210/rcsb_pdb/goodsell-gallery-023 Integrative illustration for coronavirus outreach (2020) PLoS Biol 18: e3000815 doi: 10.1371/journal.pbio.3000815.

Right: Zika Illustration by David S. Goodsell, RCSB Protein Data Bank. doi: 10.2210/rcsb_pdb/goodsell-gallery-015