Immunology Services

OVERVIEW

Immunology Services (IS) is a research service unit of the Wisconsin National Primate Research Center that specializes in measuring antigen-specific cellular responses in nonhuman primates (NHPs) and antibody-dependent cell-mediated cytotoxicity (ADCC) in NHPs and humans.

IS performs standardized ELISpot and flow cytometry-based immune assays, and conducts vaccination experiments. In addition to routine tests, IS offers consultation on the design of flow cytometry experiments and develops custom immune-assays to meet the specific aims of collaborating investigators. Primate Center investigators and other NIH-supported researchers receive priority attention from IS, but anyone is welcome to contact us. Learn how we support investigators at Immunology Services Case Studies.

We provide the following services:

• ELISpot assays to measure antigen-specific T cell and plasmablast responses.
• Intracellular Cytokine assays to measure antigen-specific T cell responses.
• Multicolor flow cytometric phenotype analysis (up to 17 parameters).
• BSL3 level-cell sorting by multicolor flow cytometry (up to 8 parameters).
• Monoclonal antibody production from supernatants of hybridomas.
• Fluorochrome conjugation to purified monoclonal antibodies.
• SIV or HIV-specific ADCC assays.
• Vaccination experiments in nonhuman primates.

STANDARDIZED FLOW CYTOMETRY STAINING PANELS

Immunology Services has standardized staining panels to monitor the phenotype and functional capability of various T cell subsets, including memory and naive T cell populations, follicular helper T cells, and regulatory T cells. We also quantify plasmablast cells, myeloid and plasmacytoid dendritic cells, classical, non-classical and intermediate monocytes. Our standardized assays to measure the frequency and functional capacity of various NK cells subsets have been published in Cytometry A (see links below).

• Activation Panel for Rhesus Macaque NK Cell Subsets (pdf)
• Functional Analysis of Natural Killer Cell Subsets in Macaques (pdf)
• Ex Vivo Analysis of SIV Infected Cells by Flow Cytometry (pdf)

We developed a staining panel to identify and quantify neutrophil progenitors in rhesus macaques.

• Neutrophil progenitor populations of rhesus macaques (pdf)

To request service, please contact the appropriate Immunology Services staff member, below.

WNPRC Immunology Services Price List (pdf)

WNPRC Immunology Services publications

RELATED LINKS

• Design an experiment on a WNPRC Immunology Services instrument: Fluorofinder sign in
• BioLegend Multicolor Staining Guide

Return to WNPRC Research Services