The Immunology Services (IS) Unit offers comprehensive support for pre-clinical studies at the WNPRC. We provide expert consultation on study design, prepare all biosafety and animal use protocols, and assemble rhesus macaque, cynomolgus macaque, or marmoset cohorts tailored to your research needs. Our team coordinates all aspects of sample collection, expertly isolates lymphocytes from blood and tissues, and manages sample shipments.
Our state-of-the-art flow cytometry facility includes a BSL3-housed cell sorter, making it ideal for infectious disease studies in nonhuman primates. With extensive experience in ELISA and flow cytometry-based assays, our staff delivers reliable, high-quality data. We have recently expanded our capabilities with a dedicated monoclonal antibody isolation core that can isolate antibodies from nonhuman primates or virtually any other animal species.
We welcome inquiries from all investigators.
Learn how we support investigators at Immunology Services Case Studies.
Supported services include:
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- Expert consultation on pre-clinical study design
- Comprehensive sample collection coordination and management
- Isolation of lymphocytes and serum/plasma from blood and tissues
- Development of custom immune assays tailored to specific research needs
- Measurement of antigen-specific T cell responses
- Multicolor flow cytometric phenotype analysis (up to 25 parameters)
- BSL3 level-cell sorting by multicolor flow cytometry (up to 18 parameters)
- Isolation of antigen-specific monoclonal antibodies
STANDARDIZED FLOW CYTOMETRY STAINING PANELS
Immunology Services has standardized staining panels to monitor the phenotype and functional capability of various T cell subsets, including memory and naive T cell populations, follicular helper T cells, and regulatory T cells. We also quantify plasmablast cells, myeloid and plasmacytoid dendritic cells, classical, non-classical and intermediate monocytes. Our standardized assays to measure the frequency and functional capacity of various NK cell subsets have been published in Cytometry A (see links below).
- Activation Panel for Rhesus Macaque NK Cell Subsets (pdf)
- Functional Analysis of Natural Killer Cell Subsets in Macaques (pdf)
- Ex Vivo Analysis of SIV Infected Cells by Flow Cytometry (pdf)
We developed a staining panel to identify and quantify neutrophil progenitors in rhesus macaques.
ANTIGEN-SPECIFIC MONOCLONAL ANTIBODY ISOLATION
We offer a cutting-edge service for isolating monoclonal antibodies from individual B cells using our novel SMART-RACE-based approach. Unlike traditional methods that struggle with diverse or poorly characterized immunoglobulin genes, our innovative cloning method eliminates the need for complex primer mixtures, enhancing recovery rates of heavy and light chain genes from single cells. This versatile platform is suitable for isolating antibodies from virtually any species—from mice and primates to non-traditional models like ferrets and pigs—making it ideal for researchers developing novel diagnostic tools, therapeutic antibodies, or research reagents.
Download our white paper describing the service: monoclonal antibody white paper.
To request service, please contact the appropriate Immunology Services staff member below.
| NAME | ||
|---|---|---|
| IMMUNOLOGY SERVICES UNIT HEAD | Dr. Dawn Dudley | dmdudley@wisc.edu |
| ANTIBODY CLONING | Dr. Matt Reynolds | matt.reynolds@wisc.edu |
| FLOW-CYTOMETRY FACILITY USAGE | Kim Weisgrau | weisgrau@primate.wisc.edu |
WNPRC Immunology Services Price List 2025-2026 (pdf)
RELATED LINKS
- Design an experiment on a WNPRC Immunology Services instrument: Fluorofinder sign in
- BioLegend Multicolor Staining Guide
Return to WNPRC Research Services